Shortening guide RNA markedly improves specificity of CRISPR-Cas nucleases

A simple adjustment to a powerful gene-editing tool may be able to improve its specificity. Massachusetts General Hospital investigators have found that adjusting the length of the the guide RNA component of the synthetic enzymes called CRISPR-Cas RNA-guided nucleases can substantially reduce the occurrence of off-target DNA mutations.

Fuente : http://www.eurekalert.org/pub_releases/2014-01/mgh…



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