Purification, culture and multi-lineage differentiation of zebrafish neural crest cells

The neural crest (NC) is a unique cell population associated with vertebrate evolution. For the first time, these multipotent cells were isolated from zebrafish embryos and maintained and differentiated in culture. The NC progenitors were differentiated into multiple neural crest lineages, contributing to neurons, glial cells, smooth muscle cells, melanocytes, and chondrocytes. Functional cell behavior assays indicated that retinoic acid had profound effect on NC cell morphology and differentiat…

Fuente : http://www.eurekalert.org/pub_releases/2014-02/sfe…